The mapping of linear B‐cell epitope regions in the extracellular parts of the desmoglein 1 and 3 proteins: recognition of immobilized peptides by pemphigus patients' serum autoantibodies
Identifieur interne : 002099 ( Main/Exploration ); précédent : 002098; suivant : 002100The mapping of linear B‐cell epitope regions in the extracellular parts of the desmoglein 1 and 3 proteins: recognition of immobilized peptides by pemphigus patients' serum autoantibodies
Auteurs : Hajnalka Szabados [Hongrie] ; Szilvia B Sze [Hongrie] ; Pálma Sill [Hongrie] ; Sarolta Kárpáti [Hongrie] ; Ferenc Hudecz [Hongrie] ; Katalin Uray [Hongrie]Source :
- Journal of Peptide Science [ 1075-2617 ] ; 2013-02.
Abstract
Desmosomal transmembrane glycoproteins desmogleins (Dsg) 1 and 3 are targets of life‐threatening autoimmune blistering disorders such as Pemphigus vulgaris (PV) and Pemphigus foliaceus (PF). In these diseases, pemphigus autoantibodies are produced against Dsg1 and Dsg3 proteins. The autoantibodies bind to these transmembrane elements leading to a loss of desmosomal cell–cell adhesion and clinically, to the presence of blisters and erosions. Identification, characterization, and detailed analysis of the binding sites of autoantibodies have an outstanding importance in understanding the immunopathology of the disease and also in the design of novel diagnostics. Here, we describe the localization of the B‐cell epitope regions of Dsg1 and Dsg3 proteins' extracellular parts recognized by IgG‐type serum autoantibodies of patients with PV and PF. In our study, overlapping pentadecapeptides were synthesized on hydroxypropyl methacrylate pins based on the results of in silico predictions. To detect the interaction between the serum autoantibodies and the immobilized synthetic peptides, modified Enzyme Linked Immunosorbent Assay (ELISA) was performed with pin‐attached peptides testing the serum samples of ten patients and four healthy donors. We identified five possible epitope regions (aa86‐110, aa196‐220, aa226‐250, aa326‐340, and aa486‐520) within the extracellular part of the Dsg1 and four possible epitope regions (aa64‐78, aa330‐344, aa375‐399, and aa446‐460) within that of the Dsg3 protein sequence using these methods. Our data showed that serum autoantibodies of patients, previously identified as Dsg1 and Dsg3 positive, are able to recognize continuous linear epitope regions of both Dsg1 and Dsg3 proteins using pin‐bound overlapping peptides in modified ELISAs. Copyright © 2013 European Peptide Society and John Wiley & Sons, Ltd.
Pemphigus specific epitope regions were identified in desmoglein 1 and 3 proteins with pin‐bound synthetic 15‐mer peptides and modified ELISAs by testing serum antibodies from patients and healthy donors. The serum antibodies of the healthy individuals did not show any specific recognition. Not only conformational but also linear epitope regions are spread in the entire extracellular region of the desmoglein 1 protein.
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DOI: 10.1002/psc.2476
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In these diseases, pemphigus autoantibodies are produced against Dsg1 and Dsg3 proteins. The autoantibodies bind to these transmembrane elements leading to a loss of desmosomal cell–cell adhesion and clinically, to the presence of blisters and erosions. Identification, characterization, and detailed analysis of the binding sites of autoantibodies have an outstanding importance in understanding the immunopathology of the disease and also in the design of novel diagnostics. Here, we describe the localization of the B‐cell epitope regions of Dsg1 and Dsg3 proteins' extracellular parts recognized by IgG‐type serum autoantibodies of patients with PV and PF. In our study, overlapping pentadecapeptides were synthesized on hydroxypropyl methacrylate pins based on the results of in silico predictions. To detect the interaction between the serum autoantibodies and the immobilized synthetic peptides, modified Enzyme Linked Immunosorbent Assay (ELISA) was performed with pin‐attached peptides testing the serum samples of ten patients and four healthy donors. We identified five possible epitope regions (aa86‐110, aa196‐220, aa226‐250, aa326‐340, and aa486‐520) within the extracellular part of the Dsg1 and four possible epitope regions (aa64‐78, aa330‐344, aa375‐399, and aa446‐460) within that of the Dsg3 protein sequence using these methods. Our data showed that serum autoantibodies of patients, previously identified as Dsg1 and Dsg3 positive, are able to recognize continuous linear epitope regions of both Dsg1 and Dsg3 proteins using pin‐bound overlapping peptides in modified ELISAs. Copyright © 2013 European Peptide Society and John Wiley & Sons, Ltd.</div><div type="abstract">Pemphigus specific epitope regions were identified in desmoglein 1 and 3 proteins with pin‐bound synthetic 15‐mer peptides and modified ELISAs by testing serum antibodies from patients and healthy donors. The serum antibodies of the healthy individuals did not show any specific recognition. Not only conformational but also linear epitope regions are spread in the entire extracellular region of the desmoglein 1 protein.</div></front></TEI><affiliations><list><country><li>Hongrie</li></country><region><li>Hongrie centrale</li></region><settlement><li>Budapest</li></settlement></list><tree><country name="Hongrie"><noRegion><name sortKey="Szabados, Hajnalka" sort="Szabados, Hajnalka" uniqKey="Szabados H" first="Hajnalka" last="Szabados">Hajnalka Szabados</name></noRegion><name sortKey="B Sze, Szilvia" sort="B Sze, Szilvia" uniqKey="B Sze S" first="Szilvia" last="B Sze">Szilvia B Sze</name><name sortKey="Hudecz, Ferenc" sort="Hudecz, Ferenc" uniqKey="Hudecz F" first="Ferenc" last="Hudecz">Ferenc Hudecz</name><name sortKey="Hudecz, Ferenc" sort="Hudecz, Ferenc" uniqKey="Hudecz F" first="Ferenc" last="Hudecz">Ferenc Hudecz</name><name sortKey="Karpati, Sarolta" sort="Karpati, Sarolta" uniqKey="Karpati S" first="Sarolta" last="Kárpáti">Sarolta Kárpáti</name><name sortKey="Sill, Palma" sort="Sill, Palma" uniqKey="Sill P" first="Pálma" last="Sill">Pálma Sill</name><name sortKey="Uray, Katalin" sort="Uray, Katalin" uniqKey="Uray K" first="Katalin" last="Uray">Katalin Uray</name><name sortKey="Uray, Katalin" sort="Uray, Katalin" uniqKey="Uray K" first="Katalin" last="Uray">Katalin Uray</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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